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citR [2019-02-05 18:32:30]
transcriptional repressor of citA
Molecular weight
35.44 kDa
Function
regulation of the minor citrate synthase
Product
transcriptional repressor (LysR family)
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
1,020,073 → 1,020,948
The protein
Expression and Regulation
Biological materials
Mutant
GP1283 Δ(citA)::aphA3, available in Jörg Stülke's labGP1753 Δ(citR citA)::aphA3, the resistance cassette can be cut out by introducing the cre-rekombinase into the chromosom of B. subtilis, available in Jörg Stülke's labGP2360 Δ(citR citA)::erm, the resistance cassette can be cut out by introducing the cre-rekombinase into the chromosom of B. subtilis, available in Jörg Stülke's labBKE09430 (ΔcitR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCTATTCTCCCTCTGA, downstream forward: _UP4_TAGGAGCAACCAAAACGCCTBKK09430 (ΔcitR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCTATTCTCCCTCTGA, downstream forward: _UP4_TAGGAGCAACCAAAACGCCT Expression vectors
pGP2264 (N-terminal His-tag, purification from E. coli, in pETM-11), available in Jörg Stülke's labpGP1023 (C-terminal Strep-tag, purification from E. coli, in pGP574), available in Jörg Stülke's labpGP1029 (N-terminal Strep-tag, purification from B. subtilis, in pGP380), available in Jörg Stülke's labpGP1030 (C-terminal Strep-tag, purification from B. subtilis, in pGP382), available in Jörg Stülke's labpGP2260 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's labpGP2262 (integration into ganA, expression under the control of the xylose-inducible PxylA promoter in B. subtilis, in pGP888), available in Jörg Stülke's lab References
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